Beilstein J. Org. Chem.2023,19, 1957–1965, doi:10.3762/bjoc.19.146
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Keywords: automated synthesis; catching-by-polymerization; gene assembly; longoligonucleotide; synthetic biology; Introduction
Long oligodeoxynucleotides (ODNs) are segments of DNAs extending beyond one hundred nucleotides (nt). Emerging research areas such as synthetic biology [1][2], protein
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Graphical Abstract
Figure 1:
Workflow for the construction, verification, and expression of the 800 bp GFP gene from chemically ...